Journal: Bioresources and Bioprocessing

Article Title: Box-Behnken optimized copper oxide nanoparticles from Thymus vulgaris potentiate efficacy against multidrug-resistant bacterial pathogens and exhibit anticancer activity

doi: 10.1186/s40643-026-01008-5

Figure Lengend Snippet: Comprehensive Evaluation of TE-CuONPs Anticancer Activity and Apoptotic Mechanism in MCF-7 Breast Cancer Cells. Concentration-dependent cytotoxicity and mechanistic analysis. a , b MTT dose–response curves against MCF-7 breast cancer cells and normal human skin fibroblasts (HSF). c , d Representative phase-contrast micrographs (200 × magnification) of MCF-7 cells after 24 h treatment at respective IC₅₀ concentrations, showing c minimal morphological changes with TE treatment versus d characteristic apoptotic features with TE-CuONPs treatment, including cell shrinkage (red arrows), membrane blebbing (yellow arrows), chromatin condensation, and cellular detachment. e Flow cytometric scatter plots (FSC-H vs. SSC-H) demonstrating consistent cell populations across treatments (10,000 events gated per sample). ( f ) Schematic proposal for anticancer mechanisms. g Annexin V-FITC/propidium iodide (PI) dual-staining flow cytometric analysis

Article Snippet: MCF-7 breast adenocarcinoma cells (ATCC HTB-22) and normal human skin fibroblasts (HSF, CRL-2522) were obtained from Nawah Scientific (Cairo, Egypt) and cultured in RPMI-1640 medium (Gibco, USA) supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C in 5% CO2.

Techniques: Activity Assay, Concentration Assay, Membrane, Staining

Journal: Materials

Article Title: Modification of Natural Clays with Magnetite to Provide Boosted Antimicrobial Properties and Chemopreventive Activity Against Melanoma

doi: 10.3390/ma18204759

Figure Lengend Snippet: Cytotoxicity assessment against human normal fibroblasts (BJ cell line) and human melanoma cells (A375 cell line) using MTT assay after exposure to 24 h extracts of the tested materials; control—cells incubated in a polypropylene extract (negative control of cytotoxicity); * statistically significant differences compared to the control ( p < 0.05, one-way ANOVA followed by Dunnett’s post hoc test).

Article Snippet: Human normal skin fibroblasts (BJ cell line, CRL-2522TM) and human melanoma cells (A375 cell line, CRL-1619TM) obtained from the American Type Culture Collection (ATCC-LGC Standards, Teddington, UK) were utilized in the cell culture experiments.

Techniques: MTT Assay, Control, Incubation, Negative Control